Application

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PL Scramblase 1 in 10 µg of human erythroleukemia K562 and Jurkat cell lysate.
Immunocytochemistry Analysis: A representative lot detected endogenous PLSCR1 in paraformaldehyde-fixed, saponin-permeabilized HT1080 cells (25 µg mAb/mL) by confocal fluorescence microscopy (Zhou, Q., et al, (2000) 95(8):2593-2599).
Immunocytochemistry Analysis: A representative lot detected endogenous PLSCR1 in formaldehyde-fixed, Triton X-100-permeabilized HT1080 cells (5 µg mAb/mL) by confocal fluorescence microscopy (Wiedmer, T., et al, (2003) Biochemistry. 42(5):1227-1233).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human PLSCR1 constructs from lysates (25 µg mAb/0.5 mL lysate) of transfected murine SVT2 cells (Wiedmer, T., et al, (2003) Biochemistry. 42(5):1227-1233).
Western Blotting Analysis: A representative lot detected human PLSCR1 in lysates from varous human cell lines (PMID 10753839, 12564925, 15308560, 16091359).

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

This Anti-PL Scramblase 1 Antibody, clone 4D2 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of PL Scramblase 1 .

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phospholipid scramblase 1 (UniProt O15162; also known as Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1) is encoded by the PLSCR1 (also known as MMTRA1B) gene (Gene ID 5359) in human. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via an ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

Immunogen

Recombinant protein corresponding to human PL Scramblase 1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed